The Human DLL1 ELISA quantitates Hu DLL1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu DLL1. Principle of the method The Human DLL1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.Delta-like protein 1 (Dlk1, DLL1) is a transmembrane protein characterized by epidermal growth factor (EGF)-like repeats. DLL1, which is also known as preadipocyte factor 1 (pref-1) because of its ability to inhibit preadipocyte differentiation, regulates the differentiation of several other cell types. It is a ligand for Notch receptor. It blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
|2°C to 8°C|
|Plasma, 33 μL; Serum, 33 μL; Supernatant, 100 μL|
|1 hr. 20 min.|
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