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Thermo Scientific™ DNase I, RNase-free: PROMO

Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.

Manufacturer:  Thermo Scientific™ EN0525

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Catalog No. FEREN0525PM

This item has been discontinued and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Description

Description

Highlights

  • Recombinant enzyme
  • Purified from non-animal host with a lower level of intrinsic RNases

Applications

  • Preparation of DNA-free RNA
  • Removal of template DNA followingin vitrotranscription
  • Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
  • DNA labeling by nick-translation in conjunction with DNA Polymerase I
  • Studies of DNA-protein interactions by DNase I, RNase-free footprinting
  • Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used

Note

DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.

Specifications

Specifications

1U/μL
E.coli cells with a cloned gene encoding bovine DNase
1000U
metal chelators, transition metals (e.g., Zn) in millimolar concentrations, SDS (even at concentrations less than 0.1%), reducing agents (DTT and beta-mercaptoethanol), ionic strength above 50-100 mM
29 kDa monomer
Documents
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