Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 405, Invitrogen™

Donkey Polyclonal Antibody

Supplier:  Thermo Scientific A48257

Catalog No. PIA48257

Description

To minimize cross-reactivity, the donkey anti-mouse IgG whole antibodies have been cross-adsorbed against serum proteins from bovine, goat, chicken, guinea pig, hamster, horse, sheep, rabbit, rat, and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically Specificity: This antibody binds to heavy chains on mouse IgG and light chains on all mouse immunoglobulins. This antibody does not bind non-immunoglobulin mouse serum proteins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep.

Mouse IgG (H+L) Highly Cross-Adsorbed Polyclonal antibody specifically detects Mouse IgG (H+L) Highly Cross-Adsorbed in Mouse samples. It is validated for Immunocytochemistry, Immunofluorescence

Specifications

• Antigen: IgG (H+L) Highly Cross-Adsorbed
• Classification: Polyclonal
• Conjugate: Alexa Fluor Plus 405
• Host Species: Donkey
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Mouse
• Form: Liquid

• Applications: Immunocytochemistry, Immunofluorescence
• Concentration: 2 mg/mL
• Formulation: proprietary buffer with 0.016% Bromonitrodioxane, 0.016% Methylisothiazolone; pH 6.5
• Immunogen: Gamma Immunoglobins Heavy and Light chains
• Quantity: 1 mg
• Primary or Secondary: Secondary
• Content And Storage: 4° C, store in dark
• Isotype: IgG