Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 405, Invitrogen™

Donkey Polyclonal Secondary Antibody

Supplier:  Thermo Scientific A48257

Catalog No. PIA48257

Description

To minimize cross-reactivity, the donkey anti-mouse IgG whole antibodies have been cross-adsorbed against serum proteins from bovine, goat, chicken, guinea pig, hamster, horse, sheep, rabbit, rat, and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically Specificity: This antibody binds to heavy chains on mouse IgG and light chains on all mouse immunoglobulins. This antibody does not bind non-immunoglobulin mouse serum proteins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep.

To minimize cross-reactivity, the donkey anti-mouse IgG whole antibodies have been cross-adsorbed against serum proteins from bovine, goat, chicken, guinea pig, hamster, horse, sheep, rabbit, rat, and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically Specificity: This antibody binds to heavy chains on mouse IgG and light chains on all mouse immunoglobulins. This antibody does not bind non-immunoglobulin mouse serum proteins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep.

Specifications

• Antigen: Mouse IgG (H+L) Highly Cross-Adsorbed
• Classification: Polyclonal
• Conjugate: Alexa Fluor Plus 405
• Host Species: Donkey
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Mouse
• Form: Liquid

• Applications: Immunocytochemistry
• Concentration: 2 mg/mL
• Formulation: proprietary buffer with 0.016% Bromonitrodioxane, 0.016% Methylisothiazolone; pH 6.5
• Immunogen: Gamma Immunoglobins Heavy and Light chains
• Quantity: 1 mg
• Primary or Secondary: Secondary
• Content And Storage: 4° C, store in dark
• Isotype: IgG