Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mixes

Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.

Supplier:  Thermo Scientific™ K9011

 View more versions of this product

Catalog No. FERK9011


Edge
Explore available promotions
Add to cart

Description

Description

Thermo Scientific DreamTaq Hot Start PCR Master Mix, available in colorless and green formats, is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq Buffer, magnesium, and dNTPs.

The master mix allows easy reaction setup and fewer pipetting steps, with only primers, template, and water needing to be added. The DreamTaq Hot Start Green PCR Master Mix includes a density reagent and two tracking dyes for convenient direct loading of PCR products on a gel.

DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases. DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step. With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases.

DreamTaq Hot Start PCR Master Mix is formulated to enhance productivity through:

  • Better reaction outcomes
    • Higher yield of target amplicons from low template amounts
    • Increased reaction specificity due to reliable hot-start technology
    • Wider range of amplicon lengths with routine amplification of genomic DNA fragments up to 6 kb
  • Enhanced convenience
    • Room temperature reaction setup
    • Minimized optimization of Mg2+ concentration and of primer annealing temperatures due to optimized reaction buffer
    • Simplified workflow and minimized pipetting steps with 2X master mix format
    • Direct loading of PCR products on a gel with green format

Applications

  • Generation of PCR products for TA cloning
  • Routine PCR
  • Colony PCR
  • Genotyping
  • RT-PCR

Recommended Storage

Store at -20C.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

• 2X DreamTaq Hot Start PCR Master Mix, 4 x 1.25 mL
• Nuclease-free water, 4 x 1.25 mL

Store at -5°C to -30°C.
DreamTaq DNA Polymerase
Dry Ice
2X
200 Reactions
SuperMix or Master Mix
Low
Standard
Hot-start PCR
Built-In Hot Start
3'-A
Colorless
Product Suggestions

Product Suggestions

SDS
Documents

Documents

Product Certifications
Promotions

Promotions

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.