Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ Dual-Light™ Luciferase & β-Galactosidase Reporter Gene Assay System

Rapid and sensitive detection of firefly luciferase and E. coli β-galactosidase in a single extract aliquot for gene expression assays.

Specifications

Product Type β-Galactosidase/Luciferase Reporter Gene Assay System
For Use With (Application) Enzyme Assay
For Use With (Equipment) Luminometer (Microplate), Luminometer (Tube-Based)
Detection Method Chemiluminescent
Format Liquid
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
T1003
Encompass
View Documents
Invitrogen™
T1003
200 Assays This item has been discontinued and is no longer available. Please call customer service for assistance: 1-800-766-7000. N/A
T1005
Encompass
View Documents
Invitrogen™
T1005
600 Assays This item has been discontinued and is no longer available. Please call customer service for assistance: 1-800-766-7000. N/A
Description

Description

  • Detection of firefly luciferase and β-galactosidase in same sample simplifies normalization of transfection efficiency
  • Wide dynamic range of β-galactosidase and luciferase assays enables accurate measurement of both enzymes from femtogram to nanogram range
  • Assay sensitivity is 100- to 1,000-fold better than either the isotopic/non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric/fluorescent assays for β-galactosidase, providing greater sensitivity than competing assay technologies
  • Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions
  • Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes
  • Assay can be completed in about one hour, providing fast assay turnaround
Easy-to-Use
Light signal from each enzymatic reaction is measured sequentially in a luminometer with automatic injectors or other instrumentation in which light emission measurements can be performed within a short period. First, luciferase reporter enzyme activity is quantitated with an enhanced luciferase reaction. Following a 30-60 minute incubation and addition of a light-emission accelerator, β-galactosidase reporter enzyme activity is determined with Galacton-Plus™ substrate. Both assays are combined into one simple assay using only one extract aliquot for greater convenience and precision. The entire assay is completed in less than one hour.
 
Wide Dynamic Range
The wide dynamic range of this dual assay enables accurate measurement of luciferase and β-galactosidase concentrations over seven orders of magnitude, from the femtogram to nanogram range. Colorimetric and isotopic reporter gene assays cannot rival the dynamic range of the Dual-Light system. We have formatted this assay for tube or microplate luminometer, with injection capability.
 
Specifications
  • Compatible Cells: Mammalian Cells, Yeast Cells
  • Detection Method: Chemiluminescent
  • For Use With Equipment: Luminometer (Microplate), Luminometer (Tube-Based)
  • Form: Liquid
  • Format: 96-well plate
  • High Throughput Compatibility: High Throughput-Compatible
  • Readout: End Point
  • Sample Type (Specific): Cell Cultures
  • Substrate: Galacton-Plus, Luciferin
  • Substrate Properties: Chemical Substrate
  • Substrate Type: Beta-Gal Substrate, Luciferase Substrate
Specifications

Specifications

β-Galactosidase/Luciferase Reporter Gene Assay System
Luminometer (Microplate), Luminometer (Tube-Based)
Liquid
Enzyme Assay
Chemiluminescent
Safety and Handling

Safety and Handling

SDS
Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit