Invitrogen™ Dynabeads™ SARS-CoV-2 Spike Ig Total ELISA Kit

The Dynabeads™ SARS-CoV-2 Spike Ig Total ELISA Kit is a solid‐phase sandwich enzyme‐linked immunosorbent assay (ELISA) designed to detect and quantify the level of human SARS-CoV-2 IgG, IgM, and IgA in serum and plasma samples. The kit can be used for both qualitative and quantitative assays.

Catalog Number	Mfr. No.	Quantity
18020D	Invitrogen™ 18020D	96 reactions
18020DFIVE	Invitrogen™ 18020DFIVE	5 x 96 reactions

Description

• A solid‐phase sandwich enzyme‐linked immunosorbent assay (ELISA) designed to detect and quantify the level of human SARS-CoV-2 IgG, IgM, and IgA in serum and plasma samples
• The steps required for use are simply to pre-dilute the reagents, fill the wells of the KingFisher plastic plates, and push start on the instrument
• After the run, the sample is transferred to a plate compatible with the absorbance reader and the OD is read at 450 nm
• A DynaMag-96 well magnet can be used while still taking advantage of the faster binding kinetics of magnetic beads compared to the more traditional plate-bound ELISAs
• Kit contains all buffers required, as well as calibrator antibodies, HRP-conjugated detection antibodies, substrate solution, beads, and stop solution sufficient for one 96-well plate
• Dynabeads SARS-CoV-2 Spike beads can be purchased separately (18100D) for other uses such as the optimization of unique conditions
• Using Dynabeads and automation drastically improves reproducibility, limits hands on time, and reduces operating errors
• The intra-assay CV value is typically <5% (range 2.8–3.2%), while the inter-assay CV is <15% (range 3.9–9.3%)
• Kit can be used for both qualitative and quantitative assays
• One of the fastest ELISAs for SARS-CoV-2 serology
• For research use only, not for use in diagnostic procedures

Specifications

• Certifications/Compliance: ISO 9001
• Product Type: SARS-CoV-2 ELISA Ig Total Kit
• For Use With (Equipment): KingFisher™ Flex or Apex Instruments, DynaMag™-96 Side Skirted, Magnetic 96-Well Separator, ELISA Plate Reader (450 nm)
• Diameter (Metric) Bead: 2.7 μm
• High-throughput Compatibility: Yes, on KingFisher™ Flex or Apex instruments

• Detection Method: Absorbance at 450 nm
• For Use With (Application): Binding of human immunoglobulins (IgG, IgM, IgA) towards SARS-CoV-2 (Spike trimer)
• Shipping Condition: Wet ice (2° to 8°C)
• Material: Monosized, superparamagnetic polystyrene magnetic beads

For research use only. Not for use in diagnostic procedures.