Sigma Aldrich Dynabind? 10 Million Compound Dna-Encoded Library

Catalog No.	Quantity
DYNA0025VL	5 Vial
DYNA0022VL	2 Vial

Catalog No. DYNA0025VL Supplier Sigma Aldrich Supplier No. DYNA0025VL

Description

MilliporeSigma Sigma Organics products encompass a wide range of quality reagents, solvents, catalysts, and building blocks for organic synthesis. From benchtop discovery to process development and scale-up, Sigma Organics solutions are built to meet the needs of synthetic chemists.

An off-the-shelf kit containing 2 or 5 vialsEach vial is for testing a single conditionA detailed user guide with an easy to follow protocol for using the kitAnalysis instructions for using the web portal to analyze the nextgen sequencing dataThis is a DNA-encoded library of 10 million small molecule compounds created by DyNAbind that can be used for drug discovery. Each compound is created using combinatorial chemistry to form trimers with over 200 building blocks at each of three positions. Each building block is tagged with a unique DNA barcode. When the building blocks are connected through amide bonds, the DNA is also combined forming a double-stranded DNA barcode for each compound. The compound is attached through an amide bond to a C6 linker which is then attached to an oxygen in the 5' phosphate on the DNA.All the compounds fit in a single microcentrifuge tube. The compounds can be incubated with a target of interest that is attached to beads. After washing, the compounds bound to the target can be detected. The DNA barcodes are amplified using PCR and then detected using nextgen sequencing. The sequencing data can be loaded to a secure portal on our website for analysis. This will generate a report of the highest affinity compounds that were bound to the target.Several methods are used to QC the library. The initial single building blocks with their attached DNA barcodes are tested by LC-MS. PCR is used to monitor library assembly, and at completion the library is tested by nextgen sequencing to ensure reasonable compound distribution. The cost of the library includes generating a list of 50 hits or up to 60 hits if there are ties. All the hit structures will be revealed at no additional charge. There are also no additional licensing fees for using the compounds for research and development or manufacturing. This kit saves time since it can be bought and used without the negotiation required for licensing other DEL technologies.Analysis PortalMore information about DyNAbindProduct of DyNAbind GmbHLabel License: ForR and D use only. Not for drug, household, or other uses. There are no restrictions, additional agreements, or fees required from Merck KGaA or from DyNAbind for using the identified compounds for any purpose.

Specifications

• Melting Point: 8.5°C
• Molecular Formula: C2H8N2
• Quantity: 5 Vial
• Molecular Weight (g/mol): 60.1 g/mol