Thermo Scientific™ DyNAmo cDNA Synthesis Kit

Get flexibility in your choice of priming strategy, including random, oligo(dT), and gene-specific primers, with this cDNA synthesis kit for two-step RT-qPCR applications.

Manufacturer:  Thermo Scientific™ F470L

Catalog No. F470L

Description

DyNAmo cDNA Synthesis Kit is intended for cDNA synthesis in two-step reverse transcription-quantitative PCR (RT-qPCR) applications, where amplicons are usually around 100 bp in length. This kit can be used in conjunction with any qPCR master mix.

The DyNAmo cDNA Synthesis Kit includes a M-MuLV RNase H⁺ reverse transcriptase and all other necessary reagents for cDNA synthesis prior to qPCR. Either total RNA, messenger RNA, viral RNA or in vitro transcribed RNA can be used as a template for reverse transcription. The kit includes both random primers and oligo(dT) primers as separate vials, offering users flexibility in their choice of priming strategy, including the option to use gene-specific primers. Reagents deliver the specified number of reactions to a final reaction volume of 20 µL.

The DyNAmo cDNA Synthesis Kit includes all necessary reagents for cDNA synthesis from various RNA sources. Total RNA, messenger RNA, viral RNA or in vitro transcribed RNA can all be used as a template for reverse transcription.

• Specifically optimized for quantitative RT-PCR
• Broad dynamic range: up to 1µg of RNA
• Reliable results with different RNA sources: mRNA, total RNA, viral RNA and in vitro transcribed RNA
• Flexible: applicable with all priming options for cDNA synthesis
• Easy to use: short protocol, 2x mix

Includes:

M-MuLV RNase H+ reverse transcriptase (includes RNase inhibitor), 2x RT buffer (includes dNTP mix and 10 mM MgCl₂), Random hexamers (300 ng/µL), Oligo(dT)15 primer (100 ng/µL).

Recommended for:

cDNA synthesis for qRT-PCR

Specifications

• For Use With (Application): cDNA synthesis for qRT-PCR
• Product Type: cDNA synthesis for qRT-PCR
• Storage Requirements: Store at -20°C.

• Includes: M-MuLV RNase H+ reverse transcriptase (includes RNase inhibitor), 2x RT buffer (includes dNTP mix and 10 mM MgCl2), Random hexamers (300 ng/µL), Oligo(dT)15 primer (100 ng/µL).
• Quantity: 20 reactions