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Thermo Scientific™ DyNAmo cDNA Synthesis Kit

Get flexibility in your choice of priming strategy, including random, oligo(dT), and gene-specific primers, with this cDNA synthesis kit for two-step RT-qPCR applications.

Specifications

For Use With (Application) cDNA synthesis for qRT-PCR
Includes M-MuLV RNase H+ reverse transcriptase (includes RNase inhibitor), 2x RT buffer (includes dNTP mix and 10 mM MgCl2), Random hexamers (300 ng/µL), Oligo(dT)15 primer (100 ng/µL).
Product Type cDNA synthesis for qRT-PCR
Storage Requirements Store at -20°C.
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Products 1
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
F470L
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Thermo Scientific™
F470L
20 reactions This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
Description

Description

DyNAmo cDNA Synthesis Kit is intended for cDNA synthesis in two-step reverse transcription-quantitative PCR (RT-qPCR) applications, where amplicons are usually around 100 bp in length. This kit can be used in conjunction with any qPCR master mix.

The DyNAmo cDNA Synthesis Kit includes a M-MuLV RNase H+ reverse transcriptase and all other necessary reagents for cDNA synthesis prior to qPCR. Either total RNA, messenger RNA, viral RNA or in vitro transcribed RNA can be used as a template for reverse transcription. The kit includes both random primers and oligo(dT) primers as separate vials, offering users flexibility in their choice of priming strategy, including the option to use gene-specific primers. Reagents deliver the specified number of reactions to a final reaction volume of 20 µL.

Highlights

  • Broad dynamic range -up to 1 µg of RNA
  • Reliable results with different RNA sources -mRNA, total RNA, viral RNA, and in vitro transcribed RNA
  • Flexible -all priming options for cDNA synthesis
  • Convenient to use -Short protocol
  • Optimized 2X mix for cDNA synthesis
Specifications

Specifications

cDNA synthesis for qRT-PCR
M-MuLV RNase H+ reverse transcriptase (includes RNase inhibitor), 2x RT buffer (includes dNTP mix and 10 mM MgCl2), Random hexamers (300 ng/µL), Oligo(dT)15 primer (100 ng/µL).
cDNA synthesis for qRT-PCR
Store at -20°C.
SDS
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