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Thermo Scientific™ DyNAmo SYBR Green 2-Step qRT-PCR Kit

Perform reliable RNA quantification with this 2-step RT-qPCR Kit.

Manufacturer:  Thermo Scientific™ F430L

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Catalog No. F430L

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Description

Description

DyNAmo SYBR Green 2-Step RT-qPCR Kit includes all the necessary reagents for cDNA synthesis and SYBR Green qPCR. The reverse transcriptase in the kits is M-MuLV RNase H+, which provides higher sensitivity for qPCR than RNase H- reverse transcriptases. The RNase H activity in the RT enzyme facilitates annealing of PCR primers to the cDNA by degrading the RNA template from the RNA-cDNA hybrid before the PCR step. The performance of the qPCR step is based on a hot start version of a modified Thermus brockianus (Tbr) DNA polymerase.

Highlights

  • Broad dynamic range: up to 1 µg of RNA
  • Reliable results with different RNA sources: mRNA, total RNA, viral RNA and in vitro transcribed RNA
  • Flexible: two-step system, all priming options
  • Optimized 2x mixes for both cDNA synthesis and qPCR
  • Specific and sensitive: uniquely engineered Tbr DNA polymerase
  • Convenient to use: 2x mixes and short protocols
Specifications

Specifications

Profiling gene expression, Quantification of RNA or DNA targets, Microbial detection, Viral load determination
cDNA synthesis:;M-MuLV RNase H+ reverse transcriptase (includes RNase inhibitor) , 2x RT buffer (includes dNTP mix and MgCl2, random hexamers (300 ng/µl), oligo(dT)15 primer (100 ng/µL).;qPCR:;2x master mix (containing hot start version of modified Therm
100 RT Rxns (200 qPCR Rxns)
Recommended storage temperature -20°C. Stable for six months from the packaging date.
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