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E4BP4 (NFIL3) Mouse anti-Human, Alexa Fluor 647, Clone: MABA223, eBioscience Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  LIFE TECHNOLOGIES 51981242

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Catalog No. 51-981-242


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Description

Description

Description: This MABA223 monoclonal antibody recognizes human E4BP4 (NFIL3), a basic leucine zipper transcription factor first identified for its binding to the promoters of the adenovirus E4 protein and human IL-3. E4BP4 is involved in the transcriptional control of the mammalian circadian clock, motor neuron growth and survival, regulation of von Willbrand factor expression, and osteoblast function. In addition, it is required for the development of NK and NKT cells as well as CD8a+ dendritic cells. Although relatively high levels of mRNA are reported in resting NK and NKT cells, studies at eBioscience have observed expression of E4BP4 protein in lymphocytes only after activation. Applications Reported: This MABA223 antibody has been reported for use in flow cytometric analysis. Applications Tested: This MABA223 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product No. 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at www.thermofisher.com/flowprotocols. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL.

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).
Specifications

Specifications

E4BP4 (NFIL3)
Monoclonal
5 μL/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Q16649
NFIL3
IgG1, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
4783
Flow Cytometry
MABA223
Alexa Fluor 647
NFIL3
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
SDS
Documents
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