Thermo Scientific™ EasyPep™ MS Sample Prep Kits

Generate high quality proteomic samples for MS analysis in less than four hours using optimized EasyPep MS Sample Prep kits.

Supplier:  Thermo Scientific™ A45734

Catalog No. PIA45734

Description

EasyPep Mini MS Sample Prep Kits enable efficient and reproducible processing of cultured mammalian cells and tissues for proteomic MS analysis. The kit contains pre-formulated buffers, MS-grade enzyme mix, peptide clean-up columns, and an optimized protocol to generate MS-compatible peptide samples in less than three hours. Kits are optimized to process protein samples from 10–100 μg with a high yield of MS-ready peptides.

• Complete—includes pre-formulated reagents for lysis through digestion, peptide clean-up columns, and an optimized protocol for up to 20 samples
• Optimized—streamlined protocol and reagents minimize the number of steps and time time needed to process samples
• Flexible—reagents and protocol have been verified using cells, plasma, and tissue samples for 10 to 100 μg samples
• Time-saving—sample processing time reduced from more than one day to less than three hours
• Compatible—final preparation is ready for direct MS analysis and other downstream applications, including TMT labeling

The sample preparation of peptides for MS analysis is complex, with numerous steps and varied protocols, resulting in sample variability in both quality and quantity. This kit has been designed to improve reproducibility while saving hands-on and processing time. The number of steps and processing time have been reduced through the addition of Universal Nuclease to reduce viscosity from nucleic acids (replacing sonication), a rapid 'one pot' reduction/alkylation solution for cysteine modification (carbamidomethylation, + 57.02), and a trypsin/Lys-C protease mix for more complete digestion. In addition, the kit includes Peptide Clean-up columns and buffers to prepare detergent-free peptide samples for MS analysis. The optimized reagents and protocol produce high-quality peptides that are compatible with TMT labeling and other downstream applications, or are ready for MS analysis.

Specifications

• Description: Maxi Sample Prep Kit
• Product Type: Sample Preparation Kit
• Content And Storage:
  • Lysis Solution, 25 mL, store at 4°C
  • Universal Nuclease, 25 kU, store at -20°C
  • Reduction Solution, 7 mL, store at 4°C
  • Alkylation Solution, 7 mL, store at 4°C
  • Enzyme Reconstititution solution, 7 mL, store at 4°C
  • Pierce™ Trypsin/Lys-C Protease Mix, MS Grade, 8 x 100 μg, store at -20°C
  • Digestion Stop Solution, 7 mL, store at 4°C
  • Peptide Clean-Up Columns, 8 Ea., store at 4°C
  • Wash Solution A, 40 mL, store at 4°C
  • Wash Solution B, 3 x 27 mL, store at 4°C
  • Elution Solution, 2 x 20 mL, store at 4°C
  • Low Protein Binding Collection Tubes, 2 mL, 40 Ea., store at 4°C
    
    
• Shipping Condition: Wet Ice
• Final Product Type: Peptides
• Workflow Step: Protein Digestion

• For Use With (Equipment): Mass Spectrometer
• Product Line: EasyPep™
• Label or Dye: Unlabeled
• Starting Material: Protein Samples
• Detection Method: Mass Spectrometry
• Quantity: 8 Reactions