EBI3 (IL-27 subunit) Mouse anti-Human, PerCP-eFluor 710, Clone: ebic6, eBioscience
Mouse Monoclonal Antibody
Manufacturer: Invitrogen 46735842
Description: This ebic6 monoclonal antibody reacts with human Epstein-Barr Virus Induced Gene 3 (EBI3). EBI3 can be expressed and secreted as monomers or homodimers, and was first identified in virally-infected B cells. It is also believed to be secreted as a homodimer by cells of the trophoblast, where it may play a role in immune tolerance during pregcy. Additionally, EBI3 is a component of two of the four members of the interleukin (IL)-12 family of heterodimeric cytokines. EBI3 was first found to heterodimerize with p28 (IL-30) to form IL-27, which is secreted by antigen-presenting cells in response to pro-inflammatory stimuli. IL-27 has been shown to have both pro-inflammatory and anti-inflammatory effects. It influences the commitment of CD4+ T-cells toward the Th1 lineage by inducing the expression of the T-bet transcription factor and the upregulation of IL-12R beta 2. Its anti-inflammatory functions include the suppression of Th2 and Th17 proliferation and differentiation. More recently, EBI3 was also found to dimerize with the IL-12 subunit, p35 to form IL-35. The biology of IL-35 is incompletely understood, but it is believed to be unique among the IL-12 family members in its expression and function. It is produced by regulatory T cells, and appears to have immunoregulatory functions.IL-35 has been found to suppress the proliferation and function of effector T cells, and has also been observed to induce a regulatory phenotype on naive CD4+ cells cultured in its presence. ELISA results from the culture of human dendritic cells stimulated shown above suggests that at least some of the EBI3 detected by flow is dimerized with p28 to form IL-27. Testing with ebic6 in the context of IL-35 is currently in process. For more information, please contact technical support. Applications Reported: This ebic6 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This ebic6 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This antibody can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 bandpass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered. EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35 (1-3). Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naive but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.
|EBI3 (IL-27 subunit)|
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|IL-27B, IL27B; EBV-induced gene 3 protein; Epstein-Barr virus induced gene 3; IL-27 subunit beta; IL27 subunit; IL35 subunit; cytokine receptor; epstein-Barr virus-induced gene 3 protein; interleukin-27 subunit beta|
|4° C, store in dark, DO NOT FREEZE!|
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