R&D Systems™ Recombinant EBOV GP Protein

Zaire ebolavirus Glycoprotein

Supplier:  R&D Systems™ 9016EB100

Catalog No. 90-16E-B100

Description

Measured by its binding ability in a functional ELISA. When Recombinant Viral EBOV GP is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CLEC10A/CD301 (Catalog # 4888-CL ) that produces 50% of the optimal binding response is approximately 20-100 ng/mL.

Specifications

• Accession Number: Q05320
• Formulation: Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
• Molecular Weight (g/mol): M.W. (Observed): 17-24 kDa and 105-120 kDa, reducing conditions; M.W. (theoretical): 69 kDa
• Source: Chinese Hamster Ovary cell line, CHO-derived viral EBOV GP protein Z. ebolavirus GP1 (Ile33-Arg501) (N-terminus) Accession # Q05320 Z. ebolavirus GP2 (Glu502-Gln650) (N-terminus) Accession # Q05320 HHHHHH (C-terminus)
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: GP
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Expression System: Chinese Hamster Ovary cell line, CHO-derived Z. ebolavirus GP1 (Ile33-Arg501) Accession # Q05320, N-terminus; Z. ebolavirus GP2 (Glu502-Gln650) Accession # Q05320; HHHHHH, C-terminus
• Protein: EBOV GP

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 3160774
• Quantity: 100 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Common Name: EBOV GP
• Biological Activity: Measured by its binding ability in a functional ELISA. When Recombinant Viral EBOV GP is immobilized at 2μg/mL (100μL/well), the concentration of Recombinant Human CLEC10A/CD301 that produces 50% of the optimal binding response is approximately 20-100ng/mL.
• Species: Virus
• Reconstitution: Reconstitute at 500 μg/mL aq. soln..
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.