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Edvotek™ Cancer Gene Detection
SCI_ED

Track cancer formation and genetic patterns.

Supplier:  Edvotek™ 115

Catalog No. S66828


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Includes: Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, & Disposable Pipets.

Requires :
  • Horizontal gel electrophoresis apparatus
  • Hotplate
  • Micropipets Balance Water bath UV transilluminator
  • 250mL flasks
  • All necessary safety equipment
  • Distilled or deionized water


Description

Description

Immortality through uncontrolled cell division is a characteristic of cancer cells. The p53 gene is a tumor suppressor gene which prevents this. Mutations in this gene are present in more than 50% of cancers. Testing people for mutations in their p53 gene can indicate an increased risk in developing cancer. These tests raise intriguing ethical questions for both the individual tested and the family of an individual who chooses to be tested. In this experiment, students determine a pedigree for a family suspected to be carriers of mutations in their p53 genes. A DNA test indicates their likelihood of developing cancer.
Specifications

Specifications

Students determine a pedigree for a family suspected to be carriers of mutations in their p53 genes
8 Gels
45 min.
Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), Practice Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, and Disposable Pipets
Horizontal electrophoresis system, Power supply, Micropipettes: 5-50 μL (optional), White light box, and Microwave or hot plate
Room temperature stable. Storage of Ready-to-Load QuickStrip™ samples in the refrigerator is recommended.
SDS
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