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Invitrogen™ ELISA-Light™ Immunoassay System with CSPD™ and Sapphire-II™ Substrate/Enhancer Solution Non-distribution product as customer accommodation.

Ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.

Manufacturer:  Invitrogen™ T1023

Catalog No. T1023



Description

Description

  • Heterogeneous assay format increases sensitivity and dynamic range and reduces interference by test compounds in the detection portion of the assay (as compared with homogeneous assay formats)
  • Chemiluminescent immunoassay detection allows greater sensitivity and improved dynamic range as compared to radioimmunoassays and immunoassays that employ colorimetric or fluorescent detection
  • High sensitivity and wide dynamic range permit sensitive detection with fewer assays, because sample dilution to fit the dynamic range of the assay is unnecessary
  • A variety of ready-to-use substrate formulations lets the user precisely optimize signal intensity to the exact requirements of an assay
  • The kinetics of the glow-assay provides a window during which measurements may be performed, facilitating HTS applications where assay automation is used
  • Chemiluminescent immunoassay detection provides versatile applications such as sandwich immunoassays, competitive immunoassays, and DNA probe capture assays
  • Non-radioactive immunoassays eliminate concerns over use of radioisotopes
Extend the Limits of Detection
The ELISA-Light system offers an excellent combination of high sensitivity and throughput for assays measured in a luminometer. The ELISA-Light system significantly extends the limits of detection of most immunoassays previously performed with colorimetric and isotopic methods.
 
Multiple Substrate/Enhancer Formulations
The kit contains reagents optimized for chemiluminescent ELISAs employing alkaline phosphatase labels and is available with a choice of ready-to-use chemiluminescent substrate formulations containing CSPD or CDP-Star™ substrate with Sapphire-II or Emerald-II™ luminescence enhancers. Multiple substrate/enhancer formulations provide options that let the user optimize specific assays or luminometers. The specific formulation that will provide the highest sensitivity for a specific assay can vary. Therefore, an ELISA sampler kit containing all four substrate/enhancer formulations is available to facilitate assay development.
 
Specifications
  • Detection Method: Chemiluminescent
  • For Use With (Equipment): Microplate Reader
  • Label or Dye: AP Alkaline Phosphatase
Specifications

Specifications

Immunoassays employing alkaline phosphatase enzyme label.
96-well plate
Immunoassay System
Store at 2° to 8°C
Chemiluminescent
Assay buffer, I-Block Reagent, substrate and enhancer
1000 assays
Documents
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