ELK1 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA587504

Catalog No. PIPA587504

Description

Immunogen sequence: EVAGCSTEDC PPQPEVSVTS TMPNVAPAAI HAAPGDTVSG KPGTPKGAGM AGPGGLARSS RNEYMRSGLY STFTIQSLQP QPPPHPRPAV VLPSAAPAGA AAPPSGSRST SPSPLEACLE AEEAGLPLQV ILTPPEAPNL KSEELNVEPG LGRALPPEVK V; Positive Samples: HeLa, Jurkat, HT-29, K-562, Mouse liver, Rat brain; Cellular Location: Nucleus

ELK1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. ELK1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by ELK1. ELK1 appears to be a direct target of activated MAP kinase. Biochemical studies indicate that ELK1 is a good substrate for MAP kinase, the kinetics of ELK1 phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block ELK1 activation in vivo. ELK1 is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for ELK1.

Specifications

• Antigen: ELK1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ELK1
• Gene Symbols: Elk1
• Immunogen: Recombit fusion protein containing a sequence corresponding to amino acids 90-250 of human ELK1
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry, Western Blot
• Concentration: 2.1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: A4GTP4, P19419, P41969
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 13712, 2002, 314436
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid