EMD Millipore Amicon™ Bioseparations Stirred Cells† Available on GSA/VA Contract for Federal Government customers only.

Concentrate, desalt, and purify aqueous biological solutions

Manufacturer: EMD Millipore 5124

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Catalog No. 5124

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Required Accessories : Magnetic stirrer required for operation.

    Specifications

    Autoclavable Yes
    Capacity (Metric) 10 to 400mL
    Capacity (English) 0.33 to 13.5 oz.
    Concentration 0.05 to 10g%
    Diameter (English) Outside 2.9 in.
    Diameter (Metric) Outside 7.6cm
    Includes Clear pressurized vessel with integral magnetic stirring bar, nylon cap and tube fitting assembly, polysulfone body and membrane holder, acetal/polysulfone stirrer, silicone O-rings and black aluminum stand, connections for 0.25 in. dia. pressure tubing
    For Use With (Application) Protein purification, protein concentration, protein desalting
    Filtration Area 41.8cm2
    Height (English) 6.1 in.
    Height (Metric) 15.5cm
    Length (English) 4.3 in.
    Length (Metric) 11cm
    Material Nylon cap and tube fitting assembly, Polysulfone cylinder and membrane holder, Acetal and Polysulfone stirring assembly, Silicone Rubber O-rings, Polyethelene pressure tube, Tygon tubing filtrate tube, Anodized Aluminum or Nylon retaining stand
    Model 8400
    Volume (Metric) Hold Up 1500μL
    Width (Metric) 11cm
    Width (English) 4.3 in.
    Capacity 0.33 to 13.5 oz., 10 to 400mL
    Dimensions (L x W x H) 4.3 x 4.3 x 6.1 in. (11 x 11 x 15.5cm)

    Process from 3mL up to 400mL of biological solutions using either concentration or diafiltration operating modes.

    • Sturdy construction; autoclavable Consist of clear pressurized vessel with integral magnetic stirring bar, nylon cap and tube fitting assembly, polysulfone body and membrane holder, acetal/polysulfone stirrer, silicone O-rings, and black aluminum stand
    Concentration Mode
    • Gas pressure is applied directly to ultrafiltration cell Solutes above membrane molecular weight cutoff are retained in cell, while water and solutes below cutoff pass into filtrate
    Diafiltration Mode
      Ultrafiltration cell is connected to auxiliary reservoir containing diafiltrate solution; reservoir is then gas pressurized Fluid volume and macrosolute concentration remain constant as filtrate is replaced by diafiltrate solution Provides a simple means for rapid microsolute exchange
    • Typically used as a substitute for dialysis

    Applications:

    Perform concentration, desalting, recovery or exclusion; Recover monoclonal antibodies and growth factors from tissue culture supernatants; Concentration and buffer exchange of chromatography fractions

  • Description & Specifications

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