MilliporeSigma™ Immobilon™ Block Noise Canceling Reagents 

Minimize background in Western blots, increasing sensitivity for superior detection of even low-abundance proteins

Manufacturer:  MilliporeSigma™ WBAVDFL01

Catalog No. WBAVDFL01

Description

 

• Ready-to-use
• Protein-free, reducing nonspecific binding, especially when using phosphoantibodies or avidin-biotin systems
• Specially engineered for the EMD Millipore SNAP i.d.™ detection system, but may be used in traditional Western blotting protocols
• Suitable for the dilution of primary and secondary antibodies
• Sufficient for 19 miniblots (each 8 × 7cm blot requires at least 26mL for SNAP i.d.)
• Compatible with streptavidin antibodies
• Compatible with PVDF (Immobilon™ P, Immobilon FL) and nitrocellulose

 

 

• Block-CH reagent is compatible with Immobilon HRP substrate

• Choose from three Block reagents

• Block-CH reagent for HRP chemiluminescence detection
• Block-FL reagent for fluorescence detection
• Block-PO reagent for phosphoprotein detection via chemiluminescence or fluorescence

 

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Specifications

• For Use With (Application): Western blotting, dot/slot blotting, mass spectrometry, fluorescence assay
• Quantity: 500 mL
• Sufficient For: Nineteen 8 x 7cm miniblots

• Product Type: Block-FL Reagent
• Storage Requirements: 20°C to 25°C