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MilliporeSigma™ Immobilon™ Block Noise Canceling Reagents

Minimize background in Western blots, increasing sensitivity for superior detection of even low-abundance proteins

Manufacturer:  MilliporeSigma™ WBAVDP001

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Catalog No. WBAVDP001

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  • Ready-to-use
  • Protein-free, reducing nonspecific binding, especially when using phosphoantibodies or avidin-biotin systems
  • Specially engineered for the EMD Millipore SNAP i.d.™ detection system, but may be used in traditional Western blotting protocols
  • Suitable for the dilution of primary and secondary antibodies
  • Sufficient for 19 miniblots (each 8 x 7cm blot requires at least 26mL for SNAP i.d.)
  • Compatible with streptavidin antibodies
  • Compatible with PVDF (Immobilon™ P, Immobilon FL) and nitrocellulose



  • Block-CH reagent is compatible with Immobilon HRP substrate
  • Choose from three Block reagents
  • Block-CH reagent for HRP chemiluminescence detection
  • Block-FL reagent for fluorescence detection
  • Block-PO reagent for phosphoprotein detection via chemiluminescence or fluorescence




Western blotting, dot/slot blotting, mass spectrometry, phosphorolation assay
500 mL
Nineteen 8 x 7cm miniblots
Block-PO Reagent
20°C to 25°C
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