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MilliporeSigma™ Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant Protein, E. coli

Supplier:  MilliporeSigma™ 324725700MIU

Catalog No. 32-472-5700MI


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Description

Description

Recombinant, Elizabethkingia meningosepticum endoglycosidase F1 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and hybrid, but not complex oligosaccharides. Core fucosylation reduces activity by 50 fold. Endo F1 will hydrolyze sulfate containing high mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F and therefore is more suitable for deglycosylation of native proteins.
Specifications

Specifications

In 20mM Tris-HCl, pH 7.5.
E. coli
RUO
Recombinant
700mIU
Endoglycosidase F1
E. meningosepticum
Liquid
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