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EMD Millipore Novagen™ BL21(DE3) Competent Cells Available on GSA/VA Contract for Federal Government customers only.

Achieve greater efficiency, reproducibility and convenience with Novagen prepared competent cells. BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high-level protein expression using a T7 RNA polymerase-IPTG induction system.

Manufacturer:  Millipore Sigma 694504

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Catalog No. 69-450-4

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  • Deficient in Ion and ompT proteases, BL21 and its derivatives are ideal for many applications

    T7 expression strain
  • Lysogen of bacteriophage DE3
  • Carries copy of T7 RNA polymerase gene under control of the lacUV5 promoter
  • Suitable for production of protein from target genes cloned in appropriate T7 expression vectors, using IPTG as an inducer
Description & Specifications


Guaranteed efficiency: >2 x 107 cfu/μg
1mL (5 x 0.2mL aliquots)
Store at ≤−70deg;C, Avoid repeated freeze/thaw
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