EMD Millipore Novagen™ BL21(DE3) Singles™ Competent Cells

Manufacturer: EMD Millipore 702353

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Catalog No. 70-235-3

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    Efficiency Guaranteed efficiency: >2 x 107 cfu/μg
    Quantity 11 reactions
    Storage Requirements Store at ≤-70deg.C

    BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both�lon�and�ompT�proteases. The designation (DE3) indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the�lacUV5promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

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