BL21 is the most widely used host background and has the advantage of being deficient in both lon (1) and ompT proteases. The designation (DE3) indicates that the strain is a lysogen of ?DE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter.
Cells dispensed in 20µL volumes in 96 well polypropylene plate compatible with thermal cyclers and water baths
Wells are individually sealed and have raised rims to prevent cross-contamination
Seals can be easily pierced with standard pipet tips or removed for access
Strips of caps are also provided for reliable sealing during manipulation and storage
Groups of 24 wells can be easily split from the plate for processing smaller numbers of samples
Efficiency: >2 x 106CFU/µg
>2 × 106CFU/μg
For high-throughput transformation
Store HT96 Competent Cells, SOC Medium and Test Plasmid at −70°C or below. Store Cap Strips, Plate Lids and Reagent Reservoirs at room temperature
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