Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

EMD Millipore Novagen™ Rosetta™ Blue (DE3) Competent Cells

Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds.

Manufacturer:  Millipore Sigma 710793

 View more versions of this product

Catalog No. 71-079-3


  Check Availability
Add to cart

Specifications

Description

RosettaBlue™ host strains are NovaBlue derivatives that combine high transformation efficiency and recA endA lacI&superscript_q; mutations with enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, and GGA on a chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters. Blue/white screening is not possible with RosettaBlue(DE3) strains due to the presence of the lacZ α-peptide coding sequence in the λDE3 prophage. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
Description & Specifications

Specifications

Guaranteed efficiency: >1 x 108 cfu/μg
1mL
Store at ≤-70deg.C
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit