EMD Millipore Novagen™ Rosetta-gami™ B (DE3) pLysS Competent Cells Available on GSA/VA Contract for Federal Government customers only.

Manufacturer: EMD Millipore 711374

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Catalog No. 71-137-4

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    Specifications

    Efficiency Guaranteed efficiency: >1 x 106 cfu/μg
    Quantity 1mL
    Storage Requirements Store at ≤-70deg.C

    Rosetta-gami B strains combine the key features of BL21 (and its Tuner™ derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm. These strains are compatible with ampicillin- or spectinomycin-resistant vectors. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.

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