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EMD Millipore Novagen™ Tuner™ DE3 Competent Cells Available on GSA/VA Contract for Federal Government customers only.

Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture.

Manufacturer:  Millipore Sigma 706233

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Catalog No. 70-623-3


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Specifications

Description

Tuner™ strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture. The lac permease (lacY) mutation allows uniform entry of IPTG into all cells in the population, which produces a concentration-dependent, homogeneous level of induction. By adjusting the concentration of IPTG, expression can be regulated from very low levels up to the robust, fully induced levels commonly associated with pET vectors. Lower-level expression may enhance the solubility and activity of difficult target proteins. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Description & Specifications

Specifications

Guaranteed efficiency: >2 x 106 cfu/μg
0.4mL
Store at ≤-70deg.C
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