Mouse anti-EMI1, Clone: EMI1/1176, Novus Biologicals
Mouse Monoclonal Antibody
Manufacturer: Novus Biologicals NBP2443440.02MG
Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), ImmunofluorescenceEMI1 Monoclonal antibody specifically detects EMI1 in Human samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry-Paraffin, Immunofluorescence.
|1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide|
|Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein|
|Store at 4C.|
|It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.|
|Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence|
|Western Blot 1 - 2 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml|
|EMI1Early mitotic inhibitor 1, F-box only protein 5, F-box protein 5, FBX5F-box protein Fbx5, Fbxo31|
|Protein A or G purified|
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