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ENO1 Mouse anti-Human, Clone: GT2217, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen MA527742

Catalog No. PIMA527742

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Positive Control: 293T, A549, H1299, HCT116 Predicted Reactivity: Mouse (94%), Rat (93%), Xenopus laevis (90%), Chicken (93%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (93%), Guinea pig (95%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

ENO1 is a neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers. NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury.


0.25 mg/ml
PBS with no preservative
enolase 1, (alpha), MPB1, ENO1L1, PPH, NNE
100 μl
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Western Blot
Recombit protein encompassing a sequence within the center region of human ENO1. The exact sequence is proprietary.
Protein G
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