BioVision Inc Enolase Activity Colorimetric/Fluorometric Assay Kit: Enolase Activity Colorimetric/Fluorometric Assay Kit: Only kit on the market to measure enolase activity. Simple & rapid. Detection limit: less than 0.04 mU. 100 assays.
Manufacturer: BioVision Inc K691100
Enolase Activity Colorimetric/Fluorometric Assay Kit: Enolase (EC 18.104.22.168), also called 2-phospho-D-glycerate hydrolase or 2-phosphoglycerate dehydratase, is a key enzyme in glycolysis. It converts 2-phosphoglycerate to phosphoenolpyruvate (PEP) & also catalyzes the reverse reaction, PEP to 2-phosphoglycerate under anabolic conditions during gluconeogenesis. Iincreased activity of enolase is associated with tumor invasion and metastasis; therefore, precise measurement of enolase activity may be of great interest for tumor diagnosis and prognosis. In BioVision's Enolase Activity Assay Kit, enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is subsequently used to generate an intermediate product. The intermediate product stoichiometrically reacts with OxiRed¿ probe to generate color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm). This simple & sensitive assay Kit can detect enolase activity less than 0.04 mU in a variety of samples.
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