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EOMES Mouse anti-Human, Porcine, FITC, Clone: WD1928, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 11487742

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Catalog No. 50-112-2132


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Description

Description

Description: This WD1928 antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R. Applications Reported: This WD1928 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This WD1928 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.

TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.
Specifications

Specifications

Eomes
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
O95936
Eomesodermin homolog; EOMES; TBR2
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Porcine
Flow Cytometry
WD1928
FITC
EOMES
Liquid
EOMES
IgG1, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
100622125, 8320
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