Invitrogen™ eRF1 Monoclonal Antibody (3B6)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA549271

Catalog No. PIMA549271

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human Hela whole cell, human Jurkat whole cell, human K562 whole cell, human Raji whole cell, human HEPG2 whole cell, rat PC-12 whole cell, mouse RAW2647 whole cell. IHC: human tonsil tissue, human ovarian cancer tissue, human breast cancer tissue, human breast cancer tissue, human liver cancer tissue, human lung cancer tissue, rat kidney tissue. ICC/IF: MCF-7 cell. Flow: HEPA1-6 cell, CACO-2 cell, RH35 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors.

Specifications

• Antigen: eRF1
• Classification: Monoclonal
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and no preservative
• Gene Accession No.: P62495, Q5U2Q7, Q8BWY3
• Gene Symbols: ETF1
• Immunogen: E.coli-derived human eRF1/ETF1 recombinant protein (Position: D9-K342).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 3B6
• Conjugate: Unconjugated
• Gene: ETF1
• Gene Alias: AI463371; D5S1995; D6Ertd109e; ERF; ERF1; ETF1; Eukaryotic peptide chain release factor subunit 1; eukaryotic release factor 1; eukaryotic translation termination factor 1; polypeptide chain release factor 1; protein Cl1; RF1; sup45 (yeast omnipotent suppressor 45) homolog-like 1; SUP45L1; TB3-1
• Host Species: Mouse
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 2107, 225363, 307503
• Content And Storage: -20°C
• Form: Lyophilized