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ERK1/2 Mouse anti-Human, Mouse, Rat, Clone: 5AD13MA, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 14910880

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Catalog No. 50-112-2759


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Description

Description

Description: The 5AD13MA monoclonal antibody recognizes human, mouse, and rat extracellular signal-regulated kinases 1 and 2 (also known as ERK1/2, p44/42, or MAPK3/1). ERK1/2 belong to a family of conserved serine/threonine protein kinases known as mitogen-activated protein kinases (MAPKs) that are involved in many cellular programs such as proliferation, differentiation, motility, and survival. The ERK1/2 signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines. The primary activators of ERK1/2 are MEK1 and MEK2 which activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187 in ERK1 and ERK2, respectively. Several downstream targets of ERK1/2 have been identified, including p90RSK and the transcription factor Elk-1. ERK1/2 are negatively regulated by MAPK phosphatases as well as by chemical inhibitors of MEK including U0126 and PD98059. The ERK pathway is disrupted in many types of cancer and thus is an important target for diagnosis and treatment. Applications Reported: This 5AD13MA antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, immunohistochemical staining, and immunocytochemistry.

Applications Tested: This 5AD13MA antibody has been tested by western blot analysis of reduced cell lysate prepared from human cells, by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval, and by immunocytochemistry of MeOH-fixed cells. This antibody can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.
Specifications

Specifications

ERK1/2
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P27361, P28482, P63085, Q63844, P63086, P21708
Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2 , MAPK1, ERK2, PRKM1, PRKM2, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p44-ERK1, MAPK3, ERK1, PRKM3, p44, p42
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Mouse, Rat
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
5AD13MA
Unconjugated
MAPK1
Liquid
MAPK1, Mapk3
IgG1, kappa
25 μg
4° C
Primary
116590, 26413, 26417, 50689, 5594, 5595
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