Thermo Scientific™ Evolution™ 260 Bio UV-Visible Spectrophotometer

The Thermo Scientific™ Evolution 260 spectrophotometer offers reliable, high-quality performance and enhanced customer usability.

Manufacturer: Thermo Scientific™ 840210900

 View more versions of this product

Catalog No. 14-380-425


This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000. 


    Drift <0.0005 A/hr, 500 nm, 1.0nm SBW, 1 hr warm-up
    Electrical Requirements 100-240 V 50-60 Hz selected automatically150W maximum
    Wavelength Scan Speed <1 to 6,000 nm/min; variable
    Accuracy (Photometric) 1A: ±0.006A
    2A: ±0.010A
    Measured at 440nm using calibrated neutral density filters traceable to NIST
    Range (Photometric) -
    Resolution (Toluene/Hexane) -
    Wavelength Slew Speed -
    Dimensions (L x W x H) 62.2 x 48.6 x 27.9 cm
    Operating System Microsoft Windows™ XP embedded
    INSIGHT 2 Software is also compatible with Windows 7 and Windows 8.1 Professional Editions and can be installed on a computer attached to the instrument
    Data Interval 10, 5, 2, 1.0, 0.5, 0.2, 0.1nm
    Pharmacopoeia Compliance Testing (Guaranteed Performance Specifications)
    Resolution (toluene in Hexane): ≥1.8A
    Photometric Accuracy (60mg/L K2Cr2O7): ±0.010A
    Stray Light: ≤1%T at 198nm: KCI; ≤0.05%AT at 220nm: Nal, KI
    Wavelength Accuracy: ±0.5nm 541.9, 546.1nm Hg emission lines, ±0.8nm full range
    Wavelength Repeatability: ≤0.05nm, repetitive scanning of 546.1nm Hg emission line
    Photometric Accuracy Instrument 1A: ±0.006A
    2A: ±0.010A
    Measured at 440nm using calibrated neutral density filters traceable to NIST
    Photometric Display -0.3 - 4.0A
    Photometric Range >3.5A
    Photometric Repeatability ±0.0002A
    Scan Ordinate Modes Absorbance, % Transmittance, % Reflectance, Kubelka-Munk, log (1/R), log (Abs), Abs*Factor, Intensity
    Description Evolution 260 Bio Local Control Spectrophotometer
    Display Touchscreen LCD panel; 800 x 480; 17.8 cm (7 in. diagonal)
    Item Description Evolution 260 LC Spectrophotometer
    Keypad Sealed membrane
    Noise 0A: <0.00015A
    1A: <0.00025A
    2A: <0.00080A
    260nm, 1.0nm SBW, RMS
    Spectral Bandwidth Variable: 1.0nm; 2.0nm; AFBG microcell optimized; AFBG fiber optic optimized; AFBG materials optimized
    Certifications/Compliance ISO 9001:2008
    Weight (Metric) 14.4 kg
    Detector Type Dual Silicon Photodiodes
    Lamp Xenon Flash Lamp, 3-year warranty (7 years typical lifetime)
    Optical Design Double Beam with sample and reference cuvette positions; Application Focused Beam Geometry; Czerny-Turner Monochromator
    Baseline Flatness ±0.0010A, 200-800nm, 1.0nm SBW, smoothing
    Scan Speed <1-6000nm/min, variable
    Type Evolution 260 Bio Local Control Spectrophotometer
    Beam Geometry Double-beam with Application Focused Beam Geometry
    Wavelength Accuracy ±0.5nm (541.9, 546.1nm mercury lines)
    ±0.8nm (full range 190-1100nm)
    Wavelength Range 190 - 1,100nm

    Advance your research with reliable, absorbance results from the Thermo Scientific™ Evolution™ 260 Bio UV-Vis spectrophotometer, designed for the changing field of life science. With the choice of tablet or computer control, the Evolution 260 Bio spectrophotometer offers high-performance measurements for a variety of biological samples and measurements. Powerful software, precision optics, and an extensive line of accessories combine for a complete solution to move you from samples to answers faster. Thermo Scientific™ INSIGHT™ software, included with the Evolution 260 Bio system, offers pre-programmed assay methods for increased accuracy and convenience.

    Complete Life Science Applications

    Next generation INSIGHT software provides the streamlined convenience of easy-to-understand nucleic acid and protein analysis methods and includes dedicated modules for:

    • Nucleic Acid concentration by direct UV measurement at 260nm including DNA, ssDNA, RNA, and siRNA
    • Nucleic acid purity measurements from 260/280nm and 260/230 ratios
    • Protein analysis by direct UV measurement at 205 or 280nm
    • Most common colorimetric analysis methods including: Pierce 660nm Protein assay, Bradford, BCA and Lowry
    • Measuring the fluorescence labeling efficiency for proteins and nucleic acids
    • Measuring the microarray labeling efficiency with one or two dyes

    Customizable, Straightforward INSIGHT Software

    • Personalize your home screen for easy navigation and convenience
    • Workbook tools save time and ensure accurate results
    • CUE scripting allows creation of customized interface and simplified workflow

    Best-in-Class Performance

    • Double-beam geometry ideal for kinetics or any sample that may change over time
    • Application Focused Beam Geometry (AFBG) technology provides superior results for microcells and turbid samples
    • Xenon flash lamp requires no warm-up time allowing instant measurements
    • Electronic triggering provides the highest level of accuracy for rapid-mixing kinetics measurements
    • Precision monochromator drive delivers fast scanning data collection and wavelength accuracy Powerful Convenience at your Fingertips
    • Color, touch screen provides instrument control using built-in computer
    • Integrated keypad allows communication with INSIGHT software
    • Choice of Local or PC configurations provides flexibility and convenience

    Accessories for all your Sampling Needs

    • Wide accessory selection with various temperature control options, sample changers, sippers and solid sampling devices complete your analysis
    • Automated cell changers with Peltier temperature control increase sample throughput
    • Choice of Peltier Single Cell Holder or a Smart 8-Cell Peltier system for temperature control and sample monitoring from 0° to 100°C
    • Easy software integration gives you complete control of your measurements

    Reliable Assurance of System Performance

    • The Thermo Scientific UV Validator package provides support for system qualification and validation activities
    • Complete documentation facilitates compliance of your system with the requirements of FDA, GxP, ISO 9001:2000 and ISPE 2001 guidelines and regulations
    • CVC and Mercury Lamp accessory automated testing ensures accuracy of your data
    • Thermo Scientific INSIGHTsecurity software provides complete tools to meet 21 CFR Part 11 requirements

  • Description & Specifications
  • Accessories

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