Thermo Scientific™ Evolution™ 300 UV-Vis Spectrophotometer

The Thermo Scientific™ Evolution 300 is ideal for life science, pharmaceutical and QA/QC applications. A wide selection of accessories and a low cost of ownership make it a versatile and affordable addition to the lab.

Manufacturer: Thermo Scientific™ 10300101

 View more versions of this product

Catalog No. 14-386-406


This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000. 

Ordering Information:

All accessories are sold separately. Available software packages include VISIONliteMaterialsCalc (for material analysis), VISIONpro (standard UV-VIS), VISIONlife (Life Science), VISIONsecurity (regulated labs), VISIONlite ColorCalc Basic (XYZ, Lab, CIE), and VISIONlite ColorCalc Advanced (>40DAB/EP color check filters, Pt-Co/Alpha color, Gardner and Iodine color, wine analysis). Other accessories include cell changers, cuvet and test holders, calibration carousels, sippers, fiber optic probes, small volume sampling, and accessories for reflectance and rapid mixing.

Recommended for:

  • General Spectroscopy
  • High-resolution UV-Visible spectrscopy
  • Temperature-based investigations

Life Science

  • Nucleic acid assays
  • Protein concentration assays
  • Thermal denaturation/renaturation
  • Kinetics


  • Quantitative analysis
  • Finished product analysis
  • Measurements in the regulated laboratory


  • General Research
  • QA/QC
  • Tablet dissolution


To use the Evolution 300 UV-Vis Spectrophotometer in a regulated or quality control environment, we offer a wide selection of compliance products and services to reduce implementation time and assure system performance.


±0.004A at 1A, ±0.004A at 2A, ±0.006A at 3AÅ
±0.0015A (200-800nm), 2.0nm SBW, smoothed
21 CFR Part 11 and audit-proof IQ/OQ/PQ documentation. ISO 9001:2000
21 in.
Evolution 300 Security UV-Vis Spectrophotometer
<0.0005Abs/hr, 500 nm, 2.0 nm SBW, 2hr warm-up
15 in.
50/60 Hz
300 PC w/VisionSecurity Software
Xenon Flash Lamp
Typical lifetime: >5 years, longer if not using live signal
Warrenty period: 3 year source replacement warrenty
Modified Ebert

Modified Ebert

Double beam with sample and reference cuvette/accessory positions

Resolution (Toluene in Hexane): Peak/Trough Ratio >2.0

Photometric Accuracy (60mg/L K2Cr2O7): <±0.01 A relative to calibrated sealed standard

Stray Light: <0.13 %T at 198 nm with KCl per EP

220 nm: <0.01%T NaI (behaves identically to KI for USP)

Wavelength Accuracy: ±0.20 nm (546.11 nm Hg emission line), ±0.30 nm 190–900 nm

Wavelength Repeatability: Peak separation of repetitive scanning of Hg line source <0.10 nm

±6 A
Absorbance, % Transmittance, % Reflectance, Concentration
Absorbance, % Transmittance, % Reflectance, Concentration,
1st-4th Derivative
Variable 0.5; 1.0; 1.5; 2.0; 4.0 nm
At least Windows XP
±0.20 nm (546.11 nm Hg emission line); ±0.3 nm for 190 - 900 nm
190 to 1100nm
3800, 2400, 1200, 600, 240, 120, 60, 30, 10, 5, 1 nm/min Intelliscan
22 kg
61 cm
±0.004A at 1A, ± 0.004A at 2A, ± 0.006A at 3A
Dual-Beam; Quartz Coated
53 cm
Dual Matched Silicon Photodiodes
100/240 V 50/60 Hz
38 cm
VisionSecurity Software, User Guide and USB Cable
Computer Control
0.5 nm


0A: <0.00018 A

1A: <0.00022 A

2A: <0.00050 A

500 nm, 2.0 nm SBW, RMS

Up to 100 mm cuvettes

1A: ±0.008 A

2A: ±0.010 A

3A: ±0.018 A

>4 A
1A: ±0.0025 A
Peak/Trough >2.0 at 0.5nm SBW
1 - 3,800 nm/min

198 nm: 2.9 A KCl

220 nm: 4.2 A NaI

340 nm: 4.3 A NaNO2

100/240 V
10, 5, 2, 1, 0.5, 0.2, 0.1, 0.05 nm
Standard deviation of 10 measurements <0.05 nm
48.4 lb.
24 in.
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