Thermo Scientific™ Evolution™ 300 UV-Vis Spectrophotometer

The Thermo Scientific™ Evolution 300 is ideal for life science, pharmaceutical and QA/QC applications. A wide selection of accessories and a low cost of ownership make it a versatile and affordable addition to the lab.

Manufacturer: Thermo Scientific™ 10300101

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Catalog No. 14-386-406

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This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000. 


    Specifications

    Accuracy ±0.004A at 1A, ±0.004A at 2A, ±0.006A at 3AÅ
    Accuracy (Photometric) ±0.004A at 1A, ± 0.004A at 2A, ± 0.006A at 3A
    Baseline Flatness ±0.0015A (200-800nm), 2.0nm SBW, smoothed
    Beam Geometry Dual-Beam; Quartz Coated
    Certifications/Compliance 21 CFR Part 11 and audit-proof IQ/OQ/PQ documentation. ISO 9001:2000
    Connections RS-232
    Depth (English) 21 in.
    Depth (Metric) 53 cm
    Description Evolution 300 Security UV-Vis Spectrophotometer
    Detector Type Dual Matched Silicon Photodiodes
    Drift <0.0005Abs/hr, 500 nm, 2.0 nm SBW, 2hr warm-up
    Electrical Requirements 100/240 V 50/60 Hz
    Height (English) 15 in.
    Height (Metric) 38 cm
    Hertz 50/60 Hz
    Includes VisionSecurity Software, User Guide and USB Cable
    Item Description 300 PC w/VisionSecurity Software
    Interface Computer Control
    Lamp Xenon Flash Lamp
    Typical lifetime: >5 years, longer if not using live signal
    Warrenty period: 3 year source replacement warrenty
    Min. Data Interval 0.5 nm
    Monochromator Modified Ebert
    Noise

    Photometric:

    0A: <0.00018 A

    1A: <0.00022 A

    2A: <0.00050 A

    500 nm, 2.0 nm SBW, RMS

    Optical Design

    Modified Ebert

    Double beam with sample and reference cuvette/accessory positions

    Pathlength (Metric) Up to 100 mm cuvettes
    Pharmacopoeia Compliance Testing

    Resolution (Toluene in Hexane): Peak/Trough Ratio >2.0

    Photometric Accuracy (60mg/L K2Cr2O7): <±0.01 A relative to calibrated sealed standard

    Stray Light: <0.13 %T at 198 nm with KCl per EP

    220 nm: <0.01%T NaI (behaves identically to KI for USP)

    Wavelength Accuracy: ±0.20 nm (546.11 nm Hg emission line), ±0.30 nm 190–900 nm

    Wavelength Repeatability: Peak separation of repetitive scanning of Hg line source <0.10 nm

    Photometric Accuracy Including Standard Tolerance

    1A: ±0.008 A

    2A: ±0.010 A

    3A: ±0.018 A

    Photometric Display ±6 A
    Photometric Range >4 A
    Photometric Readout Absorbance, % Transmittance, % Reflectance, Concentration
    Photometric Repeatability 1A: ±0.0025 A
    Range (Photometric) >4A
    Resolution (Toluene/Hexane) Peak/Trough >2.0 at 0.5nm SBW
    Scan Ordinate Modes Absorbance, % Transmittance, % Reflectance, Concentration,
    1st-4th Derivative
    Scan Speed 1 - 3,800 nm/min
    Spectral Bandwidth Variable 0.5; 1.0; 1.5; 2.0; 4.0 nm
    Stray Light

    198 nm: 2.9 A KCl

    220 nm: 4.2 A NaI

    340 nm: 4.3 A NaNO2

    System Requirements At least Windows XP
    Voltage 100/240 V
    Wavelength Accuracy ±0.20 nm (546.11 nm Hg emission line); ±0.3 nm for 190 - 900 nm
    Wavelength Data Interval 10, 5, 2, 1, 0.5, 0.2, 0.1, 0.05 nm
    Wavelength Range 190 to 1100nm
    Wavelength Repeatability Standard deviation of 10 measurements <0.05 nm
    Wavelength Scan Speed 3800, 2400, 1200, 600, 240, 120, 60, 30, 10, 5, 1 nm/min Intelliscan
    Weight (English) 48.4 lb.
    Weight (Metric) 22 kg
    Width (English) 24 in.
    Width (Metric) 61 cm

    The Thermo Scientific™ Evolution 300 is ideal for life science, pharmaceutical and QA/QC applications. A wide selection of accessories and a low cost of ownership make it a versatile and affordable addition to the lab. The powerful Xenon lamp-based platform and variable bandwidth make the Evolution 300 ideal for life science, pharmaceutical and QA/QC applications. A selection of VISION software packages expand instrument capability and Smart Accessories make your analyses easier to perform. The walk-up convenience of the Xenon lamp saves valuable time and increases your lab's productivity.

    The powerful Xenon lamp-based platform and variable bandwidth make the Evolution 300 ideal for life science, pharmaceutical and QA/QC applications. A selection of VISION software packages expand instrument capability and Smart Accessories make your analyses easier to perform. The walk-up convenience of the Xenon lamp saves valuable time and increases your lab's productivity.

    Xenon Lamp

    • Provides excellent performance over the full wavelength range
    • Ready to use without instrument warmup for fast readings
    • Activates only when taking a measurement; extended lamp life lowers cost of instrument ownership
    • 3-year warranty

    Key Features

    • 5 variable spectral bandwidth settings for matching customer assays to instrument
    • Acquisition rate: 50 data points per second
    • Longlife Xenon lamp

    Optional Smart Accessories For Any Application

    • Full line available for Life Sciences and QA/QC
    • Install or remove without restarting the instrument
    • Built-in software instantly identifies and configures to accommodate accessory
    • Each accessory has a unique serial number that is recorded for comprehensive event logs and audit trail capabilities
    • Automated performance verification with CVC accessory
    • Mercury lamp accessory is available for testing and defining wavelength accuracy

    PC Control

    • Enables data collection and distribution to third party applications for advanced processing and archiving
    • Uses powerful VISION software suite to control instrument
    • Collects, stores, recalls and analyzes data

    Local and PC Control

    Combines keypad and PC control systems for maximum flexibility in labs with a variety of experimental needs

Ordering Information:

All accessories are sold separately. Available software packages include VISIONliteMaterialsCalc (for material analysis), VISIONpro (standard UV-VIS), VISIONlife (Life Science), VISIONsecurity (regulated labs), VISIONlite ColorCalc Basic (XYZ, Lab, CIE), and VISIONlite ColorCalc Advanced (>40DAB/EP color check filters, Pt-Co/Alpha color, Gardner and Iodine color, wine analysis). Other accessories include cell changers, cuvet and test holders, calibration carousels, sippers, fiber optic probes, small volume sampling, and accessories for reflectance and rapid mixing.

Recommended for:

  • General Spectroscopy
  • High-resolution UV-Visible spectrscopy
  • Temperature-based investigations

Life Science

  • Nucleic acid assays
  • Protein concentration assays
  • Thermal denaturation/renaturation
  • Kinetics

QA/QC

  • Quantitative analysis
  • Finished product analysis
  • Measurements in the regulated laboratory

Pharmaceuctical

  • General Research
  • QA/QC
  • Tablet dissolution

Note:

To use the Evolution 300 UV-Vis Spectrophotometer in a regulated or quality control environment, we offer a wide selection of compliance products and services to reduce implementation time and assure system performance.

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