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Thermo Scientific™ Evolution™ 350 UV-Vis Spectrophotometer

Rely on the Thermo Scientific™ Evolution™ 350 UV-Vis Spectrophotometer for the performance and versatility needed for your advanced analysis

Manufacturer:  Thermo Scientific™ 840310800

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Catalog No. 14-385-196

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Designed for laboratories requiring higher performance, the Thermo Scientific™ Evolution™ 350 UV-Vis Spectrophotometer’s double beam optical design delivers precision and outstanding reliability for advanced applications. From sample to final report, intuitive software quickly guides you through each step of your analysis. Comprehensive tools for data collection, processing and reporting deliver the results you need for quantitative analysis, scanning and kinetics applications. Expansive accessory choices offer flexibility to analyze almost any sample type.

A comprehensive selection of accessories and low cost of ownership make the Evolution 350 UV-Vis Spectrophotometer a versatile and affordable addition to your lab. Powerful Thermo Scientific™ INSIGHT™ Software allows you to quickly get answers and offers expanded capabilities for more complex applications.  Smart Accessories with auto-intelligence make your analyses easier to perform. The walk-up-and-use convenience of the xenon lamp saves valuable time and increases your lab's productivity.
Applications:  Academic, Chemicals, Food & Beverage, Chemicals Environmental, Industrial QA/QC, Material Science, Pharmaceuticals
Ready for the challenge
  • Rely on the Evolution 350 Spectrophotometer’s double beam optical design providing outstanding reliability, precision performance, and long-term stability for the most demanding applications
  • Optimized performance for advanced testing requirements and peak resolution capabilities with selectable bandwidths of 0.5, 1.0, 1.5, 2.0 and 4.0 nm
  • Ensure accuracy of your data with optional Calibration Validation Carousel (CVC) for automated performance verification
  • Get instant measurements and excellent performance over entire wavelength range of 190-1100 nm with xenon flash lamp
Move from samples to answer faster  
  • Easily go from sample to final report - with intuitive INSIGHT Software guiding you every step of the way  
  • Obtain results you need for quantitative analysis, scanning and kinetics applications with comprehensive software tools for data collection, processing, and reporting
  • Customize even complex methods with ease using workflow-oriented application modules
  • Enforce data security with optional INSIGHT Security Software tools to assist with achieving 21 CFR Part 11 compliance in your pharmaceutical lab
Enhance productivity with sampling flexibility
  • Speed up your analysis with Thermo Scientific™ Smart Accessories with snap-in design for convenience and consistency
  • Eliminate manual setup with auto-recognition and software integration of sippers, cells changers, and auto-sampler accessories
  • Take advantage of the Evolution 350 Spectrophotometer’s wide beam separation and large sample compartment, providing flexibility for unique accessories and advanced applications
Regulatory/Quality Control Environments:
To use the Evolution 350 UV-Vis Spectrophotometer in a regulated or quality control environment, we offer a wide selection of compliance products and services to reduce implementation time and assure system performance.
Accessory Ordering Information:
All accessories are sold separately. Other accessories include cell changers, cuvette and test holders, calibration carousels, sippers, fiber optic probes, small volume sampling, and accessories for reflectance and rapid mixing.



±0.0015 A (200–800 nm)
2.0 nm SBW, smoothed
Dual Matched Silicon Photodiodes
<0.0005 Abs/hour, 500 nm, 2.0 nm SBW, 2 hr warm-up
Academic, Chemicals, Food & Beverage, Chemicals Environmental, Industrial QA/QC, Material Science, Pharmaceuticals
0A: <0.00018 A
1A: <0.00022 A
2A: <0.00050 A
500 nm, 2.0 nm SBW, RMS
Wavelength Accuracy: ±0.3 nm 190–900 nm
Wavelength Repeatability: SD 10 meas. <0.1 nm
Absorbance Accuracy: <±0.010A at 1A, <±0.010A at 2A
Absorbance Repeatability: ±0.0025A @ 1A
Stray Light: 198 nm with KCl >2A, 220 nm with NaI >3A, 300 nm with acetone >3A, 340 nm with NaNO2 >3A Resolution: Peak/trough ratio >1.8 @ 1 nm SBW setting
±6 A
1A: ±0.0025 A
Selectable 0.5, 1.0, 1.5, 2.0, 4.0 nm
±0.20 nm (546.11 nm Hg emission line), ±0.30 nm 190–900 nm
190–1100 nm
Variable, up to 6000 nm/min
22 kg
Evolution 350 Spectrophotometer
53 x 61 x 38 cm / 20.8 x 24 x 14.9 in.
100–240 V, 50–60 Hz
Xenon Flash Lamp
Typical Lifetime: >5 years. Longer if not using live signal
Warranty Period: 3 year source replacement warranty
Modified Ebert; Double beam with sample and reference cuvette/accessory positions
1A: ±0.004 A
2A: ±0.004 A
3A: ±0.006 A
Measured at 546 nm using neutral density filters traceable to NIST. When testing instrument performance, the specification used for pass/fail determination is the sum of the instrument specification listed here and the uncertainty in the calibration data for the filter, listed on the calibration certificate.
>4 A
Absorbance, % Transmittance, % Reflectance, Kubelka-Munk,
Log(1/R), Log(Abs), ABS × Factor, Intensity, 1st–4th Derivative
198 nm: 2.4 A KCl
220 nm: 3.5 A NaI
340 nm: 4.0 A NaNO2
10, 5, 2, 1, 0.5, 0.2, 0.1, 0.05 nm
Standard deviation of 10 measurements <0.05 nm
48.5 lbs
8.5 mm
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