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Thermo Scientific™ Exonuclease I (20 U/μL)

Name: Thermo Scientific&trade; Exonuclease I (20 U/&mu;L)
Brand: Thermo Scientific&trade;
SKU: FEREN0582
Price: 515.50 USD

Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.

Supplier: Thermo Scientific™ EN0582

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Catalog No. FEREN0582

$396.65 / Each of 1

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Reg : ~~$515.50~~
The following deviations and disclosures may produce prices below stated discounts but will not trigger price reductions: Price shown reflects a temporary, promotional price reduction. No other purchase or commercial obligation required to receive the reduced price. Price may change at any time. Promotional price valid on web orders only. Your contract pricing may differ. Other exclusions apply.
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Description

Exonuclease I (ExoI) degrades single-stranded DNA in a 3'=>5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.

It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.

Highlights

Active in PCR buffers

Applications

Primer removal from PCR mixtures:
- prior to PCR product sequencing
Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus

Note

The enzyme is not suitable for removing 3'-overhangs of dsDNA.

Specifications

Concentration	20 U/μL
Enzyme	Exonuclease
Quantity	20,000 U
Product Type	Exonuclease I

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