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Gibco™ Expi293™ MembranePro™ Expression System

Name: Gibco&trade; Expi293&trade; MembranePro&trade; Expression System
Brand: Gibco&trade;
SKU: p-7075865

Expi293™ MembranePro™ Expression System

$2090.00 - $15250.00

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Specifications

Type	Expression System
Delivery Type	Virus-Like Particles (VLPs)
Cell Line	Expi293F™
Product Line	Expi293™, MembranePro™
For Use With (Application)	Membrane Protein Expression

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Catalog Number	Mfr. No.	Quantity	Price	Quantity & Availability
Catalog Number	Mfr. No.	Quantity	Price	Quantity & Availability
A25869 View Documents Promotion Details View Product Certificates View Alternatives and Save Up to	Gibco™ A25869	10 Reactions	Each for $2,090.00		Add to Cart

A25870 View Documents Promotion Details View Product Certificates View Alternatives and Save Up to	Gibco™ A25870	100 Reactions	Each for $15,250.00		Add to Cart

Description

For researchers studying G protein–coupled receptors (GPCRs) and other membrane proteins, the Expi293™ MembranePro™ Expression System delivers enriched, functional membrane proteins with high efficiency, scalability and reliability for a wide variety of low or high throughput applications.

Features of the Expi293™ MembranePro™ Expression System include:

Efficient—more than 20-fold increase in membrane protein yield compared to the adherent culture system

Scalable—easy scale-up without the need for multiple flasks

Easy to use—no enzymatic or mechanical dissociation required

In addition, benefits of MembranePro™ technology include:

Proteins are displayed on human (or other mammalian) cell membranes

Cellular quality control and mammalian post-translational processing helps produce functional protein

Proteins bud off as lipoparticles and are easily collected from the culture medium

Lipoparticles are enriched with expressed receptors

With the less labor-intensive Expi293™ MembranePro™ Expression System protocol, you will:
1. Clone your gene of interest using a TOPO™ vector
2. Transfect a susupension culture of Expi293F™ cells using Expifectamine™ 293 transfection reagent
3. Harvest lipoparticles 48 hrs after addition of enhancers using MembranePro™ Precipitation Mix
4. Store lipoparticles at -80 C or proceed to downstream assays

Note: Exi293F™ Cells (A14527) and a pEF6 V5-His TOPO™ Expression Kit (K9610-20) are required for use of this system and must be purchased separately when needed.

Specifications

Type	Expression System
Cell Line	Expi293F™
For Use With (Application)	Membrane Protein Expression

Delivery Type	Virus-Like Particles (VLPs)
Product Line	Expi293™, MembranePro™
Promoter	EF-1α

For Research Use Only. Not for use in diagnostic procedures.

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Gibco™ Expi293™ MembranePro™ Expression System

Specifications

Description

Specifications

Documents