DescriptionFor researchers studying G protein–coupled receptors (GPCRs) and other membrane proteins, the Expi293™ MembranePro™ Expression System delivers enriched, functional membrane proteins with high efficiency, scalability and reliability for a wide variety of low or high throughput applications.
Features of the Expi293™ MembranePro™ Expression System include:
In addition, benefits of MembranePro™ technology include:
With the less labor-intensive Expi293™ MembranePro™ Expression System protocol, you will:
1. Clone your gene of interest using a TOPO™ vector
2. Transfect a susupension culture of Expi293F™ cells using Expifectamine™ 293 transfection reagent
3. Harvest lipoparticles 48 hrs after addition of enhancers using MembranePro™ Precipitation Mix
4. Store lipoparticles at -80 C or proceed to downstream assays
Note: Exi293F™ Cells (A14527) and a pEF6 V5-His TOPO™ Expression Kit (K9610-20) are required for use of this system and must be purchased separately when needed.
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|Membrane Protein Expression|
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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