DescriptionFor researchers studying G proteincoupled receptors (GPCRs) and other membrane proteins, the Expi293 MembranePro Expression System delivers enriched, functional membrane proteins with high efficiency, scalability and reliability for a wide variety of low or high throughput applications.
Features of the Expi293 MembranePro Expression System include:
In addition, benefits of MembranePro technology include:
With the less labor-intensive Expi293 MembranePro Expression System protocol, you will:
1. Clone your gene of interest using a TOPO vector
2. Transfect a susupension culture of Expi293F cells using Expifectamine 293 transfection reagent
3. Harvest lipoparticles 48 hrs after addition of enhancers using MembranePro Precipitation Mix
4. Store lipoparticles at -80 C or proceed to downstream assays
Note: Exi293F Cells (A14527) and a pEF6 V5-His TOPO Expression Kit (K9610-20) are required for use of this system and must be purchased separately when needed.
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|Protein biology, protein expression, mammalian expression, membrane protein expression|
|Expi293™ MembranePro™ Expression System|
For Research Use Only. Not for use in diagnostic procedures.
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