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Gibco™ Expi293™ MembranePro™ Expression System Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Expi293™ MembranePro™ Expression System

$1550.00 - $11450.00

Specifications

For Use With (Application) Protein biology, protein expression, mammalian expression, membrane protein expression
Formulation Liquid
Product Type Expi293™ MembranePro™ Expression System
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
A25869 Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only. View Documents Gibco™
A25869
10 rxn(s) Each for $1,550.00
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A25870 Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only. View Documents Gibco™
A25870
100 rxn(s) Each for $11,450.00
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Description

Description

For researchers studying G protein–coupled receptors (GPCRs) and other membrane proteins, the Expi293™ MembranePro™ Expression System delivers enriched, functional membrane proteins with high efficiency, scalability and reliability for a wide variety of low or high throughput applications.

Features of the Expi293™ MembranePro™ Expression System include:
  • Efficient—more than 20-fold increase in membrane protein yield compared to the adherent culture system
  • Scalable—easy scale-up without the need for multiple flasks
  • Easy to use—no enzymatic or mechanical dissociation required

    In addition, benefits of MembranePro™ technology include:
  • Proteins are displayed on human (or other mammalian) cell membranes
  • Cellular quality control and mammalian post-translational processing helps produce functional protein
  • Proteins bud off as lipoparticles and are easily collected from the culture medium
  • Lipoparticles are enriched with expressed receptors

    With the less labor-intensive Expi293™ MembranePro™ Expression System protocol, you will:
    1. Clone your gene of interest using a TOPO™ vector
    2. Transfect a susupension culture of Expi293F™ cells using Expifectamine™ 293 transfection reagent
    3. Harvest lipoparticles 48 hrs after addition of enhancers using MembranePro™ Precipitation Mix
    4. Store lipoparticles at -80 C or proceed to downstream assays

    Note: Exi293F™ Cells (A14527) and a pEF6 V5-His TOPO™ Expression Kit (K9610-20) are required for use of this system and must be purchased separately when needed.

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  • Specifications

    Specifications

    Protein biology, protein expression, mammalian expression, membrane protein expression
    Liquid
    Expi293™ MembranePro™ Expression System

    For Research Use Only. Not for use in diagnostic procedures.

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