Gibco™ ExpiSf9™ Cells

Gibco™ ExpiSf9™ Cells are non-engineered derivative of Sf9 insect cells that have been adapted for high-density suspension growth in ExpiSf CD Medium.

Supplier:  Gibco™ A35243

Catalog No. A35243

Description

• Enable consistent protein expression performance for over 20 passages after thaw
• Allow you to attain yields of three times more protein per liter than other insect cells from baculovirus-infected culture as part of the ExpiSf Expression System
• Achieve maximum cell densities of 2x10⁷ cells per mL in ExpiSf CD Medium
• Exhibit fast recovery post-thaw and doubling times of approximately 24 hours
• Achieve high performance growth in multiple culture volumes from 4mL in deep-well plates to >3L in shake flasks

ExpiSf9 Cells are a core component of the ExpiSf Expression System. Frozen cells are supplied in a vial containing 1.5mL of cells at 1 x 10⁷ viable cells per mL in ExpiSf™ CD Medium and 7.5% DMSO. The cells should be thawed in ExpiSf™ CD Medium.Cells are typically grown in shake flasks on a shaker platform in a non-humidified, non-CO2 cell culture incubator. Growing ExpiSf9 cells in adherent format for routine culture is not recommended.

Specifications

• Content And Storage: 1 vial (1.5 mL volume at 1.0 x 10⁷ cells/mL), store in vapor-phase liquid nitrogen
• Cell Type: Insect Cells
• Species: S. frugiperda
• Product Type: Insect Cells

• Cell Line: Sf9, SFM Adapted
• Culture Type: Suspension Cell Culture
• Product Line: ExpiSf9™
• Shipping Condition: Dry Ice

For Research Use Only. Not for use in diagnostic procedures.