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Edvotek™ Exploring Plant Diversity with DNA Barcoding Experiment Kit
Students explore the genetic diversity of ten selected plants in this experiment. Edvotek™ Exploring Plant Diversity with DNA Barcoding Experiment Kit used to generate unique identification profiles for each plant.
Supplier: Edvotek™ 338
Includes: Instructions, PCR EdvoBeads™, Primer G Mix Concentrate, Primer LP Mix Concentrate, EdvoQuick™ DNA Ladder, Control DNA Concentrate, TE Buffer, Proteinase K, NaCl Solution, DNA Extraction Buffer, Restriction Enzyme Reaction Buffer, Restriction Enzyme Dilution Buffer, AluI Restriction Enzyme, UltraSpec-Agarose™, 10X Gel Loading Solution, 50X Electrophoresis Buffer, InstaStain™ Ethidium Bromide, FlashBlue™ Liquid Stain, Micropestles, Microcentrifuge & PCR Tubes, & Wax Beads
Requires : Thermal cycler, centrifuge, isopropanol, distilled water, electrophoresis apparatus, power supply, adjustable micropipets and tips, microwave or hot plate, waterbath, UV transilluminator.
Description
- Students isolate plant DNA and use PCR to amplify two polymorphic regions of the chloroplast genome
- Digestion of PCR products and analysis by agarose gel electrophoresis used for unique identification profiles for each plant
- For 10 Lab Groups
- DNA Isolation (2 hrs) Set-Up (30 min) DNA Digest (60 min)
- Requires wet ice shipment for next day delivery
Specifications
Instructions, DNA extraction reagents, PCR EdvoBeads™, microtubes, primers, DNA ladder, Restriction Enzyme, Restriction Enzyme Reaction and Dilution, buffers, ultrapure water, wax beads, gel loading dye, agarose, electrophoresis buffer, InstaStain™ Ethidium Bromide and FlashBlue™ stain. | |
DNA Isolation (2 hrs) Set-Up (30 min) DNA Digest (60 min) | |
Freezer storage (some components) | |
10 Lab Groups |
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