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Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin, No Weigh Format

Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin No-Weigh™ Format is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.

Manufacturer:  Thermo Scientific™ A39258

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Catalog No. PIA39258

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Thermo Scientific™ EZ-Link Sulfo-NHS-SS-Biotin enables simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution.

  • Protein labeling - biotinylate antibodies to facilitate immobilization, purification or detection
  • Cell surface labeling - biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
  • Amine-reactive - reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides
  • Cleavable - disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT; only a small sulfhydryl group remains attached to the molecule
  • Soluble - charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
  • Medium length - spacer arm (total length added to target) is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain


Store at -20°C.
DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
Chemical Labeling
Wet Ice
Sulfo-NHS Ester
Cleavable, 24.3 angstroms
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