F-Actin Mouse, Clone: NH3, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 6 publications

Supplier:  Novus Biologicals NB10064792

Catalog No. NB10064792

Description

Actin Gamma 1 Monoclonal specifically detects Actin Gamma 1 in Human, Mouse, Rat, Rabbit samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

• Antigen: F-Actin
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 1:100-1:500, Flow Cytometry 1:10, ELISA 1:10, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
• Gene Alias: actin, cytoplasmic 2, DFNA26, ACT, ACTG, actin, gamma 1, BRWS2, DFNA20
• Host Species: Mouse
• Purification Method: IgM purified
• Regulatory Status: RUO
• Gene ID (Entrez): 71
• Target Species: Human, Mouse, Rat, Rabbit
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
• Clone: NH3
• Conjugate: Unconjugated
• Gene Accession No.: P63261
• Gene Symbols: ACTG1
• Immunogen: Human monocytes and U937 cell line
• Quantity: 0.25 mg
• Primary or Secondary: Primary
• Test Specificity: Recognizes human Filamentous actin (F-actin). The binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgM