F4/80 Rat anti-Mouse, Clone: BM8, eBioscience
Rat Monoclonal Antibody
Manufacturer: Invitrogen 14480182
Description: The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160 kDa surface receptor. It belongs to the EGF-TM7 family of proteins. As such it contains seven EGF-like domains on its extracellular N-terminus, seven transmembrane spanning sequences, and an intracellular C-terminal domain showing homology to other TM7 superfamily members. The F4/80 antigen is expressed by a majority of mature macrophages and is one of the best markers for this population of cells. However, other cell types, such as peritoneal eosinophils, Langerhans cells, and some other dendritic cell subtypes, have been reported to express this antigen as well. Expression of F4/80 commences during early myeloid development in vivo and can be upregulated on BM cells stimulated in vitro with M-CSF. Some populations of macrophages, especially in the lymphoid microenvironment, may be devoid of F4/80. Applications Reported: The BM8 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of paraffin embedded tissue sections. Immunoblotting must be performed under non-reducing conditions. For immunohistochemical staining of paraffin sections, proteinase treatment is needed. It has been reported that reduction with 2-mercaptoenthanol destroys the BM8 antigen. Applications Tested: The BM8 antibody has been tested by flow cytometric analysis of mouse spleen or bone marrow cells.This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The BM8 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using pronase treatment and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
|PBS with 0.09% sodium azide; pH 7.2|
|DD7A5-7, EGF-TM7, F4/80, Gpf480, Ly71, TM7LN3|
|Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)|
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