Factor X/Xa Monoclonal Antibody (9050), Invitrogen™

Rat Monoclonal Antibody

Supplier:  Thermo Scientific MA143035

Catalog No. 01-674-523

Description

This antibody detects mouse factor X (heavy chain) and mouse factor Xa (heavy chain) by ELISA and Western blot, and cross-reacts with human factor X/Xa (Western blot only). Western blot can be performed under reducing and non-reducing conditions. Inhibitory properties: Does not inhibit PT (mouse plasma). Purity >95% by SDS-PAGE.

Factor X undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity.

Specifications

• Antigen: Factor X/Xa
• Classification: Monoclonal
• Concentration: 8 mg/mL
• Formulation: 50% water with 50% glycerol and no preservative
• Gene Accession No.: O88947, P00742
• Gene Symbols: F10
• Immunogen: Purified mouse factor X
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Western Blot
• Clone: 9050
• Conjugate: Unconjugated
• Gene: F10
• Gene Alias: Activated factor Xa heavy chain; blood coagulation factor X; Cf10; coagulation factor 10; coagulation factor X; coagulation factor X protein; F10; Factor X heavy chain; Factor X light chain; factor Xa; FX; FXA; prothrombinase; stuart factor; Stuart-Prower factor; Unknown (protein for IMAGE:8121240); unnamed protein product
• Host Species: Rat
• Purification Method: purified
• Regulatory Status: RUO
• Gene ID (Entrez): 14058, 2159
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov