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Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase Promotion

Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .

Manufacturer:  Thermo Scientific™ EF0651

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Catalog No. FEREF0651PM

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
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Description

Description

FastAP Thermosensitive Alkaline Phosphatase catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA, and nucleotides. This enzyme also removes phosphate groups from proteins.

FastAP is a novel alkaline phosphatase, which is active in all Thermo Scientific restriction enzyme buffers as well as in PCR buffers. It dephosphorylates all types of DNA ends (blunt, 5'- and 3'-overhangs) in 10 minutes at 37°C. The enzyme is inactivated in 5 minutes at 75°C . Therefore, removal of alkaline phosphatase is not required prior to ligation.

Highlights

  • Recombinant enzyme
  • Fast dephosphorylation -10 minutes at 37°C
  • Fast and complete inactivation -5 minutes at 75°C
  • Simultaneous digestion and dephosphorylation of vector DNA
  • 100% active in restriction enzyme and PCR buffers
  • One protocol for all types of DNA ends:
    • 5'-overhangs
    • 3'-overhangs
    • blunt-ends
    • single nucleotides
  • PCR clean-up in conjunction with Exo I
  • Protein dephosphorylation
Specifications

Specifications

metal chelators
E.coli cells with a cloned bacterial AP gene
1000U
Documents
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The purchase of this product allows the purchaser to use it for preparing amplified DNA fragments under a license from GE Healthcare of U.S. Patent Nos. 5,741,676 and 5,756,285 and other foreign patents.