Fc gamma RIIIA/CD16a Mouse anti-Human, Clone: HO-80, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2479540.2MG

Catalog No. NBP247954B

Description

Fc gamma RIIIA/CD16a Monoclonal antibody specifically detects Antigen in Human samples. It is validated for Flow Cytometry, Immunofluorescence.

Specifications

• Antigen: Fc gamma RIIIA/CD16a
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
• Gene Alias: CD16a, CD16a antigen, CD16FCRIIIA, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), Fc fragment of IgG, low affinity IIIa, receptor for (CD16), Fc gamma receptor III-A, FCG3, Fc-gamma receptor III-2 (CD 16), Fc-gamma receptor IIIb (CD16), Fc-gamma RIII, Fc-gamma RIIIa, Fc-gamma RIII-alpha, FCGR3, FCGRIII, FCR-10, FcRIII, FcRIIIa, IGFR3FcgammaRIIIA, immunoglobulin G Fc receptor III, low affinity III, receptor for (CD16), low affinity immunoglobulin gamma Fc region receptor III-A, neutrophil-specific antigen NA
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: It recognizes CD16 (Fcgamma RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80kDa. Two similar genes represent CD16, CD16A (Fcgamma RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fcgamma RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG2a κ

• Applications: Flow Cytometry, Immunofluorescence, CyTOF
• Clone: HO-80
• Conjugate: Unconjugated
• Formulation: PBS with No Preservative
• Gene Symbols: FCGR3A
• Immunogen: PBL s from a NK-leukemia patient
• Quantity: 0.2 mg
• Research Discipline: Astrocyte Markers, Cellular Markers, Cytokine Research, Cytoskeleton Markers, Growth and Development, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Stem Cell Markers
• Gene ID (Entrez): 2214
• Target Species: Human
• Form: Purified