Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Biotin Chromogenic Detection Kit Available on GSA/VA Contract for Federal Government customers only.

Perform chromogenic detection of biotinylated nucleic acid probes with the ThermoScientific™ Biotin Chromogenic Detection Kit.

Manufacturer:  Thermo Scientific™ K0662

 View more versions of this product

Catalog No. FERK0662


Add to cart

Description

Description

Biotin Chromogenic Detection Kit is a convenient tool for the chromogenic detection of biotinylated nucleic acid probes. The kit is optimized to reproducibly provide high sensitivity with a low background in applications, such as Southern, Northern, dot and slot blotting, and screening of viral plaques and bacterial colonies. Biotinylation of DNA/RNA probes is widely used as a safe and convenient alternative to radioactive labeling. Biotin can be incorporated into nucleic acids using various enzymatic or non-enzymatic methods, including the Biotin DecaLabel DNA Labeling Kit. 

The kit is optimized to reproducibly provide high sensitivity with a low background in applications such as Southern, Northern, dot and slot blotting, as well as screening of viral plaques and bacterial colonies. Biotinylation of DNA/RNA probes is widely used as a safe and convenient alternative to radioactive labeling. Biotin can be incorporated into nucleic acids using various enzymatic or non-enzymatic methods including the Biotin DecaLabel DNA Labeling Kit. Biotinylated probes are detected with streptavidin coupled to alkaline phosphatase (AP). Streptavidin-AP conjugates bind specifically and irreversibly to the biotin-labeled probes. The probes then are visualized using a chromogenic substrate for alkaline phosphatase BCIP/NBT, which produces a blue-purple precipitate. Therefore, visualization does not require X-ray film or other specific equipment (Fig. 1). The Biotin Chromogenic Detection Kit includes Blocking reagent and Blocking/Washing buffers which are specifically designed to minimize background, which is often associated with biotin detection in conventional systems.The positively charged SensiBlot Plus Nylon Membrane is a recommended component of this detection system to ensure the highest sensitivity of detection and the lowest background.
  • High sensitivity of detection – 0.03pg of homologous DNA in dot blot hybridization with biotin-labeled probe
  • Low background due to optimized washing and blocking procedures
  • Non-radioactive detection visualization does not require X-ray film or other specific equipment
  • Fast and convenient – ready-to-use components

Recommended for:

Southern, Northern, dot/slot blots, plaque or colony screening.

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Specifications

Specifications

Southern, Northern, dot/slot blots, plaque or colony screening.
Streptavidin-AP Conjugate;BCIP/NBT Solution;Blocking Reagent;10X Blocking/Washing Buffer;10X Detection Buffer;Detailed Protocol
30
Documents
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit