Thermo Scientific™ BseXI (BbvI) (3 U/μL)

Cut at GCAGC(8/12)^ sites with BseXI (BbvI) restriction enzyme, which performs best at 65°C in its own unique buffer (Isoschizomers: BbvI, BstV1I).

Supplier:  Thermo Scientific™ ER1452

Catalog No. FERER1452

Description

Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'			G	C	A	G	C	N8	↓		3'
3'			C	G	T	C	G	N12	↑		5'

Thermo Scientific BseXI (BbvI) restriction enzyme recognizes GCAGC(8/12)^ sites and cuts best at 65°C in its own unique buffer (isoschizomers: BbvI, BstV1I). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.

In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: Assayed using pBR322 DNA (#SD0041). Incubation at 37°C results in 10% activity. Greater than 40-fold overdigestion with BseXI may result in star activity. BseXI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis. For methylation sensitivity, refer to product specifications.

Specifications

• Concentration: 3 U/μL
• Enzyme: BseXI (BbvI)
• Sensitive to Heat Inactivation: Yes
• Type IIS RE: Yes
• Research Category: Scarless Cloning

• Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
• Compatible Buffer: Unique Buffer (10X Buffer BseXI (BbvI))
• Optimal Reaction Temperature: 65°C
• Product Type: Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures.