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Thermo Scientific™ DecaLabel DNA Labeling Kit

Get fast synthesis of radiolabeled DNA for hybridization experiments with Thermo Scientific™ DecaLabel™ DNA Labeling Kit.

Specifications

For Use With (Application) Generation of radiolabeled DNA probes for use in a variety of hybridization experiments: Southern and Northern blots, colony/plaque hybridizations, dot/slot blots and in situ hybridizations.
Includes Klenow Fragment, exo-;Decanucleotides in Reaction Buffer;Mix A (-dATP);Mix C (-dCTP);dNTP Mix;Control Template;Water, nuclease-free;Detailed Protocol
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Catalog Number Mfr. No. No. of Reactions Price Quantity & Availability  
Catalog Number Mfr. No. No. of Reactions Price Quantity & Availability  
FERK0621
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Thermo Scientific™
K0621
10 This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
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Description

Description

DecaLabel DNA Labeling Kit is an advanced system for fast synthesis of radiolabeled DNA probes of high specific activity. It's based on the improved random-primed method developed by Feinberg and Vogelstein.

The primary improvement over traditional random-primer kits involves the use of random decamers instead of hexamers to ensure more efficient annealing with DNA at 37°C. In addition, Klenow Fragment, exo- has been genetically engineered to remove exonuclease activity. The improved enzyme does not degrade the labeled probe during reaction and allows high labeling yields even from low template amounts. DNA fragments of any length can be uniformly labeled. Two labeling mixes are provided for flexibility in using either radiolabeled dATP or dCTP.

Highlights

  • High specific radioactivity of probes - greater than 1 x 109 dpm/µg DNA
  • Fast - 5 minutes at 37°C
  • High yields with Klenow Fragment, exo- no degradation of a labeled probe during reaction
  • Flexible - suitable for either radiolabeled dATP or dCTP
Specifications

Specifications

Generation of radiolabeled DNA probes for use in a variety of hybridization experiments: Southern and Northern blots, colony/plaque hybridizations, dot/slot blots and in situ hybridizations.
Klenow Fragment, exo-;Decanucleotides in Reaction Buffer;Mix A (-dATP);Mix C (-dCTP);dNTP Mix;Control Template;Water, nuclease-free;Detailed Protocol
SDS
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