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Thermo Scientific™ Eco81I (Bsu36I) Available on GSA/VA Contract for Federal Government customers only.

The Eco81I (Bsu36I) restriction enzyme recognizes CC^TNAGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: AxyI, Bse21I, Bsu36I).

Manufacturer:  Thermo Scientific™ ER0372

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Catalog No. FERER0372


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Description

Description

Lambda DNA digested with Eco81I (Bsu36I), 0.7% agarose, 2 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...C C^T N A G G...3'
  • 3'...G G A N T^C C...5' Conditions for 100% Activity:
    • 1X Buffer Tango: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
    • Incubate at 37°C
    Storage Buffer:
    • Eco81I is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol
    Ligation and Recleavage:
    • After 10-fold overdigestion with Eco81I, more than 80% of the M13mp18 DNA fragments can be ligated in a reaction mixture containing 20-40U of T4 DNA Ligase/1µg of fragments and 10% PEG
    • More than 95% of these can be recut
    Methylation Effects:
    • Dam: never overlaps-no effect
    • Dcm: never overlaps-no effect
    • CpG: never overlaps-no effect
    • EcoKI: never overlaps-no effect
    • EcoBI: may overlap-effect not determined
    Digestion of Agarose-embedded DNA:
    • Minimum 5U of the enzyme are required for complete digestion of 1µg of agarose-embedded lambda DNA in 16 hours
    Compatible Ends:
    • BbvCI, Bpu10I, Bpu1102I, HpyF3I

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  • Specifications

    Specifications

    Eco81I (Bsu36I)
    10U/μL
    2,500U
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